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Image Search Results
Journal: bioRxiv
Article Title: 2’- O -methylation of the second transcribed nucleotide within mRNA 5’ cap impacts protein production level in a cell specific manner and contributes to RNA immune evasion
doi: 10.1101/2022.02.03.478939
Figure Lengend Snippet: Relative total protein production after 72 h measured in the medium from culture of (A , B) A549 and (C , D) JAWS II cells transfected with IVT mRNAs encoding Gaussia luciferase bearing various cap analogs at their 5’ ends. Bars represent mean value ± SEM normalized to transcripts with cap0(A). Statistical significance: NS – not significant, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 (one-way ANOVA with Turkey’s multiple comparisons test), (raw data from three independent biological replicates are shown in SI Appendix, Fig. S1, each independent biological replicate consisted of three independent transfections). (E) Summary table presenting influence of cap methylation status on protein production levels in A549, JAWS II, THP-1 and 3T3-L1 cells relative to transcript with cap0(A), ↑ – increase of protein production, ↓ – decrease of protein production, double arrow means large (at least 3-time) increase/decrease, ↔ – no significant change.
Article Snippet: A549 human carcinoma lung cells (ATCC CCL-185),
Techniques: Transfection, Luciferase, Methylation
Journal: bioRxiv
Article Title: 2’- O -methylation of the second transcribed nucleotide within mRNA 5’ cap impacts protein production level in a cell specific manner and contributes to RNA immune evasion
doi: 10.1101/2022.02.03.478939
Figure Lengend Snippet: (A) Scheme of affinity purification approach used in this study. (B) Heatmap showing hierarchical clustering of proteins performed on log2 ratio values using Euclidean distances generated in Perseus software . Ratio of any protein identified and quantified was calculated in comparison to the average level in the mock samples normalized to “1.0” (Heatmaps with marked clusters are presented in SI Appendix, Fig. S4). (C) Pearson correlation coefficient for dentified interactomes for differently capped RNA for A549 and JAWS II cells (Exact correlations between identified interactomes for differently capped RNA are presented in SI Appendix, Fig. S5).
Article Snippet: A549 human carcinoma lung cells (ATCC CCL-185),
Techniques: Affinity Purification, Generated, Software, Comparison